Amino Acid Sequence of C-Terminal Fragment of Hog Pepsin
نویسندگان
چکیده
منابع مشابه
Amino-acid sequence of porcine pepsin.
As the culmination of several years of experiments, we propose a complete amino-acid sequence for porcine pepsin, an enzyme containing 327 amino-acid residues in a single polypeptide chain. In the sequence determination, the enzyme was treated with cyanogen bromide. Five resulting fragments were purified. The amino-acid sequence of four of the fragments accounted for 290 residues. Because the s...
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Attempts to apply the technique of Edman (l), for the stepwise degradation of peptides with the formation of phenylthiohydantoins (PTH), to the study of the N-terminal amino acid sequence of pepsin have resulted in only qualified success. One of the principal drawbacks of this technique arises from the fact that the reactions entailed are usually not complete. Thus, residual amounts of terminal...
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The sequence of 27 residues at the carboxyl end of the single polypeptide chain of porcine pepsin has been found to be: -Ile-Leu-Gly-Asp-Val-Phe-Ile-Arg-Gln-Tyr-Tyr-ThrVal-Phe-Asp-Arg-Ala-Asn-Asn-Lys-Val-Gly-Leu-Ala-ProVal-Ala. The peptides from which this sequence has been derived were isolated from tryptic and chymotryptic digests of pepsin and its reduced aminoethylated and trifluoracetylate...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1970
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1970.tb00948.x